Scientists at the National Institute of Animal Nutrition and Physiology (NIANP), Bengaluru, under the Indian Council of Agricultural Research (ICAR), have developed a ready-to-use, egg yolk-free solution – CRYODIL.

About CRYODIL

• Based on recent developments reported by the Indian Council of Agricultural Research (ICAR), 
• CRYODIL is an egg yolk-free semen extender developed for the cryopreservation (freezing) of buffalo semen. 

Key features

• Egg yolk-free: Unlike traditional extenders, CRYODIL does not use egg yolk, which eliminates the risk of microbial contamination. The new solution uses purified whey proteins instead.
• Extended shelf life: It can preserve buffalo semen for up to 18 months, which is a significantly longer shelf life compared to conventional extenders.
• Improved sperm motility: Tests have shown that sperm cryopreserved in CRYODIL have significantly higher "post-thaw progressive motility"—the ability to swim after being frozen and thawed—which is crucial for successful fertilization.
• Cost-effective and indigenous: CRYODIL is cheaper and easier to produce than traditional and imported commercial extenders. This fosters self-reliance for India in animal breeding technology. 

Significance and development

• Developed by NIANP: CRYODIL was created by scientists at the National Institute of Animal Nutrition and Physiology (NIANP) in Bengaluru, which operates under the Indian Council of Agricultural Research (ICAR).
• Enhances buffalo breeding: By improving the success rate of artificial insemination (AI), the solution is expected to enhance India's dairy productivity and contribute to the dairy economy. 

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